Anti-sickling activity of Ficus sycomorus leaves extract in homozygous and heterozygous sickle cell disease

Sene Mbaye; Diouf Ibrahima; Ba Fatoumata; Ba Awa; Thiam Baye Bacar; Diaw Ndeye Absatou; Sarr Mamadou; Sene Madieye; Kane Modou Oumy

doi:10.5455/OJPP.2026.v1.i1.1

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Open J Physiol Pharmacol. 2026; 1(1): 1-5

doi: 10.5455/OJPP.2026.v1.i1.1

Anti-sickling activity of Ficus sycomorus leaves extract in homozygous and heterozygous sickle cell disease

Sene Mbaye, Diouf Ibrahima, Ba Fatoumata, Ba Awa, Thiam Baye Bacar, Diaw Ndeye Absatou, Sarr Mamadou, Sene Madieye, Kane Modou Oumy.

Abstract
Background:
Sickle cell disease is a hereditary genetic disorder with autosomal recessive transmission and a notably high prevalence. Studies on some plants used in traditional medicine have shown significant in vitro anti-sickling activity.

Aim:
This study aimed to evaluate whether a hydroethanolic extract of Ficus sycomorus leaves can prevent red blood cell sickling in individuals with HbAS and HbSS genotypes.

Methods:
Ficus sycomorus leaf powder (10 g) was macerated in 100 ml of 60% ethanol. Following filtration, the macerate was concentrated using a rotary evaporator to obtain a crude dry extract. This extract was then dissolved in water to prepare two concentrations: 5 and 2.5 mg/ml. After incubating the extract solutions with blood samples from individuals with HbAS and HbSS genotypes, as well as from healthy HbAA controls, Emmel tests were performed. The percentage of sickled erythrocytes was determined using optical microscopy.

Results:
In the basal state, HbSS subjects had a higher sickle cell count (44.20% ± 0.66%) than HbAS subjects (30.99% ± 0.88%). The F. sycomorus leaf extract (EFFs) resulted in a significant reduction in the sickle cell count in subjects with HbSS, with the rate dropping from 44.20% ± 0.66% to 26.13% ± 0.88% at a concentration of 2.5 mg/ml. In HbAS subjects, the sickle cell count decreased from 30.99% ± 0.88% to 13.16% ± 0.44% at the same concentration. However, increasing the extract concentration to 5 mg/ml seemed to lessen this effect, with only a slight reduction in the preventive effect on sickling (p < 0.05 for inhibitory effect vs control).

Conclusion:
Our findings suggest that the crude hydroethanolic extract of F. sycomorus leaves exhibits promising anti-sickling properties.

Key words: Anti-sickling; Ficus sycomorus; Sickle cell disease.

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How to Cite this Article

Pubmed Style

Mbaye S, Ibrahima D, Fatoumata B, Awa B, Bacar TB, Absatou DN, Mamadou S, Madieye S, Oumy KM. Anti-sickling activity of Ficus sycomorus leaves extract in homozygous and heterozygous sickle cell disease. Open J Physiol Pharmacol. 2026; 1(1): 1-5. doi:10.5455/OJPP.2026.v1.i1.1

Web Style

Mbaye S, Ibrahima D, Fatoumata B, Awa B, Bacar TB, Absatou DN, Mamadou S, Madieye S, Oumy KM. Anti-sickling activity of Ficus sycomorus leaves extract in homozygous and heterozygous sickle cell disease. https://www.ojpponline.org/?mno=296475 [Access: February 06, 2026]. doi:10.5455/OJPP.2026.v1.i1.1

AMA (American Medical Association) Style

Mbaye S, Ibrahima D, Fatoumata B, Awa B, Bacar TB, Absatou DN, Mamadou S, Madieye S, Oumy KM. Anti-sickling activity of Ficus sycomorus leaves extract in homozygous and heterozygous sickle cell disease. Open J Physiol Pharmacol. 2026; 1(1): 1-5. doi:10.5455/OJPP.2026.v1.i1.1

Vancouver/ICMJE Style

Mbaye S, Ibrahima D, Fatoumata B, Awa B, Bacar TB, Absatou DN, Mamadou S, Madieye S, Oumy KM. Anti-sickling activity of Ficus sycomorus leaves extract in homozygous and heterozygous sickle cell disease. Open J Physiol Pharmacol. (2026), [cited February 06, 2026]; 1(1): 1-5. doi:10.5455/OJPP.2026.v1.i1.1

Harvard Style

Mbaye, S., Ibrahima, . D., Fatoumata, . B., Awa, . B., Bacar, . T. B., Absatou, . D. N., Mamadou, . S., Madieye, . S. & Oumy, . K. M. (2026) Anti-sickling activity of Ficus sycomorus leaves extract in homozygous and heterozygous sickle cell disease. Open J Physiol Pharmacol, 1 (1), 1-5. doi:10.5455/OJPP.2026.v1.i1.1

Turabian Style

Mbaye, Sene, Diouf Ibrahima, Ba Fatoumata, Ba Awa, Thiam Baye Bacar, Diaw Ndeye Absatou, Sarr Mamadou, Sene Madieye, and Kane Modou Oumy. 2026. Anti-sickling activity of Ficus sycomorus leaves extract in homozygous and heterozygous sickle cell disease. Open Journal of Physiology and Pharmacology, 1 (1), 1-5. doi:10.5455/OJPP.2026.v1.i1.1

Chicago Style

Mbaye, Sene, Diouf Ibrahima, Ba Fatoumata, Ba Awa, Thiam Baye Bacar, Diaw Ndeye Absatou, Sarr Mamadou, Sene Madieye, and Kane Modou Oumy. "Anti-sickling activity of Ficus sycomorus leaves extract in homozygous and heterozygous sickle cell disease." Open Journal of Physiology and Pharmacology 1 (2026), 1-5. doi:10.5455/OJPP.2026.v1.i1.1

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Mbaye, S., Ibrahima, . D., Fatoumata, . B., Awa, . B., Bacar, . T. B., Absatou, . D. N., Mamadou, . S., Madieye, . S. & Oumy, . K. M. (2026) Anti-sickling activity of Ficus sycomorus leaves extract in homozygous and heterozygous sickle cell disease. Open Journal of Physiology and Pharmacology, 1 (1), 1-5. doi:10.5455/OJPP.2026.v1.i1.1

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